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anti chd5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti chd5
    IHC staining with <t> anti-CHD5 </t> on human testicular spermatogenic cells
    Anti Chd5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+chd5/CHD5+Rabbit+mAb/pmc11245580-72-5-7
    Average 92 stars, based on 5 article reviews
    anti chd5 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Evaluation of CHD5 , H3K9me3 , and H4K12ac in Human Testes with Spermatogenic Maturation Arrest: A Cross-Sectional Study"

    Article Title: Evaluation of CHD5 , H3K9me3 , and H4K12ac in Human Testes with Spermatogenic Maturation Arrest: A Cross-Sectional Study

    Journal: International Journal of Fertility & Sterility

    doi: 10.22074/IJFS.2023.1996254.1451

    IHC staining with anti-CHD5 on human testicular spermatogenic cells
    Figure Legend Snippet: IHC staining with anti-CHD5 on human testicular spermatogenic cells

    Techniques Used: Immunohistochemistry

    Related Articles

    Immunohistochemistry:

    Article Title: Evaluation of CHD5 , H3K9me3 , and H4K12ac in Human Testes with Spermatogenic Maturation Arrest: A Cross-Sectional Study
    Article Snippet: .. We performed immunohistochemistry staining with anti- CHD5 (Cell Signaling, 44829S), anti- H3K9me3 (Abcam, ab8898), and H4K12ac (Abcam, ab46983) antibodies on testicular biopsy from azoospermic patients. .. Immunohistochemistry was performed according to the Starr Trek Universal HRP Detection System Protocol (Biocare Medical, US).

    Staining:

    Article Title: Evaluation of CHD5 , H3K9me3 , and H4K12ac in Human Testes with Spermatogenic Maturation Arrest: A Cross-Sectional Study
    Article Snippet: .. We performed immunohistochemistry staining with anti- CHD5 (Cell Signaling, 44829S), anti- H3K9me3 (Abcam, ab8898), and H4K12ac (Abcam, ab46983) antibodies on testicular biopsy from azoospermic patients. .. Immunohistochemistry was performed according to the Starr Trek Universal HRP Detection System Protocol (Biocare Medical, US).

    other:

    Article Title: Osteocalcin as a treatment for male reproductive disorders
    Article Snippet: Afterward, the FLIPR dye (Calcium Plus Assay Kit Dye, Molecular Devices) diluted in the same buffer supplemented with 2.5 mM probenecid, pH 7.4 was exchanged for the previous medium and incubated with the cells for an additional 30-45 minutes.



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    Image Search Results


    IHC staining with anti-CHD5 on human testicular spermatogenic cells

    Journal: International Journal of Fertility & Sterility

    Article Title: Evaluation of CHD5 , H3K9me3 , and H4K12ac in Human Testes with Spermatogenic Maturation Arrest: A Cross-Sectional Study

    doi: 10.22074/IJFS.2023.1996254.1451

    Figure Lengend Snippet: IHC staining with anti-CHD5 on human testicular spermatogenic cells

    Article Snippet: We performed immunohistochemistry staining with anti- CHD5 (Cell Signaling, 44829S), anti- H3K9me3 (Abcam, ab8898), and H4K12ac (Abcam, ab46983) antibodies on testicular biopsy from azoospermic patients.

    Techniques: Immunohistochemistry

    Representative images of CHD5 immunohistochemical staining. (A) Absence of CHD5 nuclear staining in cancer cells, intensity score 0. (B) Weak CHD5 nuclear staining in cancer cells, intensity score 1. (C) Moderate CHD5 nuclear staining in cancer cells, intensity score 2. (D) Strong CHD5 nuclear staining in cancer cells, intensity score 3. All images were obtained with an original magnification of ×400. CHD5, chromodomain helicase DNA-binding 5.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: Representative images of CHD5 immunohistochemical staining. (A) Absence of CHD5 nuclear staining in cancer cells, intensity score 0. (B) Weak CHD5 nuclear staining in cancer cells, intensity score 1. (C) Moderate CHD5 nuclear staining in cancer cells, intensity score 2. (D) Strong CHD5 nuclear staining in cancer cells, intensity score 3. All images were obtained with an original magnification of ×400. CHD5, chromodomain helicase DNA-binding 5.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: Immunohistochemical staining, Staining, Binding Assay

    Clinicopathologic characteristics of gastric cancer patients according to CHD5 expression.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: Clinicopathologic characteristics of gastric cancer patients according to CHD5 expression.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: Expressing

    Kaplan-Meier survival of patients with gastric cancer according to CHD5 expression. (A) Overall survival and (B) recurrence-free survival. CHD5, chromodomain helicase DNA-binding 5.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: Kaplan-Meier survival of patients with gastric cancer according to CHD5 expression. (A) Overall survival and (B) recurrence-free survival. CHD5, chromodomain helicase DNA-binding 5.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: Expressing, Binding Assay

    Cox multivariate analysis of overall survival.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: Cox multivariate analysis of overall survival.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: Expressing

    The RNA expression of CHD5 in gastric cancer cell lines was determined using reverse transcription-quantitative PCR. (A) GAPDH was used to normalize the CHD5 expression and HeLa was used as the reference. (B) CHD5 was upregulated by the CRISPR/Cas9 system in gastric cancer cell lines AGS, NUGC-3 and MKN45. CHD5, chromodomain helicase DNA-binding 5.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: The RNA expression of CHD5 in gastric cancer cell lines was determined using reverse transcription-quantitative PCR. (A) GAPDH was used to normalize the CHD5 expression and HeLa was used as the reference. (B) CHD5 was upregulated by the CRISPR/Cas9 system in gastric cancer cell lines AGS, NUGC-3 and MKN45. CHD5, chromodomain helicase DNA-binding 5.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: RNA Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, CRISPR, Binding Assay

    (A) Cell proliferation assay of gastric cancer cell lines AGS, NUGC-3 and MKN45, comparing CHD5- and control-transfected cells. Gastric cancer cells transfected with CHD5-plasmid have significantly decreased proliferation at 48, 72 and 96 h compared with transfected with Control-plasmid. A total of 8 samples were used for each experiment. (B) Scratch wound-healing assay of gastric cancer cell lines (AGS, NUGC-3 and MKN45), comparing CHD5- and control-transfected cells. CHD5-transfected cells have significantly less ability to heal the artificial wound than control-transfected cells at 12, 24 and 36 h. A total of 5 samples were used for each experiment. (C) Invasion assay of gastric cancer cell lines (AGS and NUGC-3), comparing CHD5- and control-transfected cells. CHD5-transfected cells demonstrated significantly less invasion than control-transfected cells. Three samples were used for each experiment. *P<0.05. CHD5, chromodomain helicase DNA-binding 5.

    Journal: Oncology Letters

    Article Title: Clinical significance of chromatin remodeling factor CHD5 expression in gastric cancer

    doi: 10.3892/ol.2019.11138

    Figure Lengend Snippet: (A) Cell proliferation assay of gastric cancer cell lines AGS, NUGC-3 and MKN45, comparing CHD5- and control-transfected cells. Gastric cancer cells transfected with CHD5-plasmid have significantly decreased proliferation at 48, 72 and 96 h compared with transfected with Control-plasmid. A total of 8 samples were used for each experiment. (B) Scratch wound-healing assay of gastric cancer cell lines (AGS, NUGC-3 and MKN45), comparing CHD5- and control-transfected cells. CHD5-transfected cells have significantly less ability to heal the artificial wound than control-transfected cells at 12, 24 and 36 h. A total of 5 samples were used for each experiment. (C) Invasion assay of gastric cancer cell lines (AGS and NUGC-3), comparing CHD5- and control-transfected cells. CHD5-transfected cells demonstrated significantly less invasion than control-transfected cells. Three samples were used for each experiment. *P<0.05. CHD5, chromodomain helicase DNA-binding 5.

    Article Snippet: The following antibodies (all at 1:1,000 dilution) were used in the present study: ACTB (Sigma-Aldrich), CHD5 (Santa Cruz Biotechnology, Inc.), p53 (DAKO), Murine Double Minute 2 (MDM2; Santa Cruz Biotechnology, Inc.), Enhancer of zeste homologue 2 (EZH2; Invitrogen), and tri-methylation at lysine 27 of histone H3 (H3K27me3; Abcam).

    Techniques: Proliferation Assay, Control, Transfection, Plasmid Preparation, Wound Healing Assay, Invasion Assay, Binding Assay